HIV drug resistance (HIVDR) testing can assist clinicians in selecting treatments. However, high complexity and cost of genotyping assays limit routine testing in settings where HIVDR prevalence has reached high levels.
The oligonucleotide ligation assay (OLA)-Simple kit was developed for detection of HIVDR against first-line non-nucleoside/nucleoside reverse transcriptase inhibitors and validated on 672 codons (168 specimens) from subtypes A, B, C, D, and AE.
The kit uses dry reagents to facilitate assay setup, lateral flow devices for visual HIVDR detections, and in-house software with an interface for guiding users and analyzing results.HIVDR analysis of specimens by OLA-Simple compared to Sanger sequencing revealed 99.6 ± 0.3% specificity and 98.2 ± 0.9% sensitivity, and compared to high-sensitivity assays, 99.6 ± 0.6% specificity and 86.2 ± 2.5% sensitivity, with 2.6 ± 0.9% indeterminate results. OLA-Simple was performed more rapidly compared to Sanger sequencing (<4 h vs. 35-72 h).
Forty-one untrained volunteers blindly tested two specimens each with 96.8 ± 0.8% accuracy.OLA-Simple compares favorably with HIVDR genotyping by Sanger and sensitive comparators. Instructional software enabled inexperienced, first-time users to perform the assay with high accuracy.
Sulforhodamine 101 |
80101 |
Biotium |
500MG |
EUR 236 |
Description: Minimum order quantity: 1 unit of 500MG |
Trap 101 |
B7084-10 |
ApexBio |
10 mg |
EUR 373 |
Trap 101 |
B7084-50 |
ApexBio |
50 mg |
EUR 1363 |
UFP-101 |
B5128-1 |
ApexBio |
1 mg |
EUR 583 |
LOXO-101 |
B6176-100 |
ApexBio |
100 mg |
EUR 696 |
Description: IC50: low nanomolar range for inhibition of all TRK family membersLOXO-101 is a tropomyosin receptor kinases (TRK) inhibitor.The TRK family of neurotrophin receptors and their neurotrophin ligands regulate neuron growth, differentiation and survival. |
LOXO-101 |
B6176-25 |
ApexBio |
25 mg |
EUR 290 |
Description: IC50: low nanomolar range for inhibition of all TRK family membersLOXO-101 is a tropomyosin receptor kinases (TRK) inhibitor.The TRK family of neurotrophin receptors and their neurotrophin ligands regulate neuron growth, differentiation and survival. |
LOXO-101 |
B6176-5 |
ApexBio |
5 mg |
EUR 128 |
Description: IC50: low nanomolar range for inhibition of all TRK family membersLOXO-101 is a tropomyosin receptor kinases (TRK) inhibitor.The TRK family of neurotrophin receptors and their neurotrophin ligands regulate neuron growth, differentiation and survival. |
GlyH-101 |
B1087-10 |
ApexBio |
10 mg |
EUR 195 |
Description: GlyH-101 is a cell-permeable glycinyl hydrazone compound that blocks CFTR with Ki of 1.4 uM. |
GlyH-101 |
B1087-5.1 |
ApexBio |
10 mM (in 1mL DMSO) |
EUR 148 |
Description: GlyH-101 is a cell-permeable glycinyl hydrazone compound that blocks CFTR with Ki of 1.4 uM. |
GlyH-101 |
B1087-50 |
ApexBio |
50 mg |
EUR 659 |
Description: GlyH-101 is a cell-permeable glycinyl hydrazone compound that blocks CFTR with Ki of 1.4 uM. |
ARP 101 |
A4433-5 |
ApexBio |
5 mg |
EUR 334 |
Description: ARP 101 is a selective MMP-2 inhibitor with IC50 value of 0.81nM. |
AT-101 |
A3196-10 |
ApexBio |
10 mg |
EUR 171 |
Description: AT101, a natural product from cottonseed with a BH3-mimetic structure, was identified as a small molecule inhibitor of Bcl-2/Bcl-xL/Mcl-1 that potently induces apoptosis in various cancer cell lines [1]. |
AT-101 |
A3196-50 |
ApexBio |
50 mg |
EUR 432 |
Description: AT101, a natural product from cottonseed with a BH3-mimetic structure, was identified as a small molecule inhibitor of Bcl-2/Bcl-xL/Mcl-1 that potently induces apoptosis in various cancer cell lines [1]. |
Sulforhodamine 101 |
B7762-100 |
ApexBio |
100 mg |
EUR 137 |
Sulforhodamine 101 |
B7762-250 |
ApexBio |
250 mg |
EUR 257 |
Sulforhodamine 101 |
B7762-500 |
ApexBio |
500 mg |
EUR 390 |
AS 101 |
B7830-5 |
ApexBio |
5 mg |
EUR 171 |
Description: Ammonium tri-chloro(dioxoethylene-O,O?-)tellurate (AS-101), an anti?IL-10 compound, is a nontoxic immunomodulator.AS101 (0.43222.5 ?g/ml) decreased IL-10 production in a dose-dependent manner. |
CUDC-101 |
A4092-10 |
ApexBio |
10 mg |
EUR 137 |
Description: CUDC-101 is a multitargeted inhibitor of histone deacetylase. It is designed to directly inhibit epidermal growth factor receptor (EGFR) and HER2 as well as class I and class II HDACs. |
CUDC-101 |
A4092-200 |
ApexBio |
200 mg |
EUR 943 |
Description: CUDC-101 is a multitargeted inhibitor of histone deacetylase. It is designed to directly inhibit epidermal growth factor receptor (EGFR) and HER2 as well as class I and class II HDACs. |
CUDC-101 |
A4092-5.1 |
ApexBio |
10 mM (in 1mL DMSO) |
EUR 158 |
Description: CUDC-101 is a multitargeted inhibitor of histone deacetylase. It is designed to directly inhibit epidermal growth factor receptor (EGFR) and HER2 as well as class I and class II HDACs. |
CUDC-101 |
A4092-50 |
ApexBio |
50 mg |
EUR 386 |
Description: CUDC-101 is a multitargeted inhibitor of histone deacetylase. It is designed to directly inhibit epidermal growth factor receptor (EGFR) and HER2 as well as class I and class II HDACs. |
CUDC-101 |
A4092-S |
ApexBio |
Evaluation Sample |
EUR 81 |
Description: CUDC-101 is a multitargeted inhibitor of histone deacetylase. It is designed to directly inhibit epidermal growth factor receptor (EGFR) and HER2 as well as class I and class II HDACs. |
UCF 101 |
C4437-10 |
ApexBio |
10 mg |
EUR 186 |
Description: IC50: 9.5 ?MUCF 101 is an inhibitor of Omi/HtrA2. Omi/HtrA2, a mitochondria serine protease with close homology to bacterial HtrA chaperones, is released from the mitochondria in response to apoptotic stimuli. |
UCF 101 |
C4437-25 |
ApexBio |
25 mg |
EUR 354 |
Description: IC50: 9.5 ?MUCF 101 is an inhibitor of Omi/HtrA2. Omi/HtrA2, a mitochondria serine protease with close homology to bacterial HtrA chaperones, is released from the mitochondria in response to apoptotic stimuli. |
UCF 101 |
C4437-5 |
ApexBio |
5 mg |
EUR 145 |
Description: IC50: 9.5 ?MUCF 101 is an inhibitor of Omi/HtrA2. Omi/HtrA2, a mitochondria serine protease with close homology to bacterial HtrA chaperones, is released from the mitochondria in response to apoptotic stimuli. |
miRZip-101 anti-miR-101 microRNA construct |
MZIP101-PA-1 |
SBI |
Bacterial Streak |
EUR 684 |
|
KHS 101 hydrochloride |
B7717-10 |
ApexBio |
10 mg |
EUR 318 |
KHS 101 hydrochloride |
B7717-50 |
ApexBio |
50 mg |
EUR 1162 |
H1F0 (Ab-101) Antibody |
1-CSB-PA010087OA101nme1HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against H1F0 (Ab-101). Recognizes H1F0 (Ab-101) from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF, ChIP; Recommended dilution: WB:1:200-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
HIST1H2AG (Ab-101) Antibody |
1-CSB-PA010389PA101nogHU |
Cusabio |
|
|
|
Description: A polyclonal antibody against HIST1H2AG (Ab-101). Recognizes HIST1H2AG (Ab-101) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200 |
CNOT2 (Ab-101) Antibody |
CSB-PA951939- |
Cusabio |
|
EUR 335 |
|
Description: A polyclonal antibody against CNOT2 (Ab-101). Recognizes CNOT2 (Ab-101) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
CNOT2 (Ab-101) Antibody |
CSB-PA951939-100ul |
Cusabio |
100ul |
EUR 316 |
|
Description: A polyclonal antibody against CNOT2 (Ab-101). Recognizes CNOT2 (Ab-101) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
Tsg 101 Polyclonal Antibody |
ES7453-100ul |
ELK Biotech |
100ul |
EUR 279 |
Description: A Rabbit Polyclonal antibody against Tsg 101 from Human/Mouse/Rat/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
Tsg 101 Polyclonal Antibody |
ES7453-50ul |
ELK Biotech |
50ul |
EUR 207 |
Description: A Rabbit Polyclonal antibody against Tsg 101 from Human/Mouse/Rat/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
CNOT2 (Ab-101) Antibody |
33291-100ul |
SAB |
100ul |
EUR 252 |
CNOT2 (Ab-101) Antibody |
33291-50ul |
SAB |
50ul |
EUR 187 |
Tsg 101 Polyclonal Antibody |
ABP56454-003ml |
Abbkine |
0.03ml |
EUR 158 |
|
Description: A polyclonal antibody for detection of Tsg 101 from Human, Mouse, Rat, Monkey. This Tsg 101 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Tsg 101 at AA range: 250-330 |
Tsg 101 Polyclonal Antibody |
ABP56454-01ml |
Abbkine |
0.1ml |
EUR 289 |
|
Description: A polyclonal antibody for detection of Tsg 101 from Human, Mouse, Rat, Monkey. This Tsg 101 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Tsg 101 at AA range: 250-330 |
Tsg 101 Polyclonal Antibody |
ABP56454-02ml |
Abbkine |
0.2ml |
EUR 414 |
|
Description: A polyclonal antibody for detection of Tsg 101 from Human, Mouse, Rat, Monkey. This Tsg 101 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Tsg 101 at AA range: 250-330 |
Anti-Tsg 101 antibody |
STJ96125 |
St John's Laboratory |
200 µl |
EUR 197 |
Description: Rabbit polyclonal to Tsg 101. |
hsa-miR-101 Primers |
MPH01016 |
ABM |
150 ul / 10 uM |
EUR 121 |
CFTR Inhibitor, GlyH-101 |
2863-25 |
Biovision |
|
EUR 756 |
CFTR Inhibitor, GlyH-101 |
2863-5 |
Biovision |
|
EUR 229 |
Monoclonal SDC1 / Syndecan 1 / CD138 Antibody (clone DL-101), Clone: DL-101 |
AMR09843G |
Leading Biology |
0.05mg |
EUR 484 |
Description: A Monoclonal antibody against Human SDC1 / Syndecan 1 / CD138 (clone DL-101). The antibodies are raised in Mouse and are from clone DL-101. This antibody is applicable in IHC-P, IHC-Fr, Flo |
Recombinant ASFV Pret-101 Protein |
VAng-2391Lsx-inquire |
Creative Biolabs |
inquire |
Ask for price |
Description: African swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996) Uncharacterized protein G1340L (Pret-101), partial, recombinant protein from E. coli. (Uniprot ID: P0CA42) |
Transmembrane Protein 101 (TMEM101) Antibody |
20-abx015121 |
Abbexa |
-
EUR 314.00
-
EUR 98.00
-
EUR 398.00
-
EUR 495.00
|
- 100 ug
- 10 ug
- 200 ug
- 300 µg
|
|
Apo B48 / 101 Antibody (Biotin) |
abx023237-1ml |
Abbexa |
1 ml |
EUR 787 |
|
Tumor Susceptibility Gene 101 Protein |
20-abx262882 |
Abbexa |
-
EUR 328.00
-
EUR 6397.00
-
EUR 230.00
|
|
|
Testis Expressed 101 (TEX101) Antibody |
abx238605-100ug |
Abbexa |
100 ug |
EUR 551 |
|
Transmembrane Protein 101 (TMEM101) Antibody |
abx331212-100ul |
Abbexa |
100 ul |
EUR 425 |
|
Transmembrane Protein 101 (TMEM101) Antibody |
20-abx325447 |
Abbexa |
|
|
|
Sulforhodamine 101 (Texas-Red) Phalloidin |
00033 |
Biotium |
300EU |
EUR 345 |
Description: Minimum order quantity: 1 unit of 300EU |
Recombinant ASFV Ken-101 Protein |
VAng-0862Lsx-inquire |
Creative Biolabs |
inquire |
Ask for price |
Description: African swine fever virus (isolate Pig/Kenya/KEN-50/1950) Uncharacterized protein G1340L (Ken-101), partial, recombinant protein from E. coli. (Uniprot ID: P0CA40) |
Recombinant ASFV Ba71V-101 Protein |
VAng-2017Lsx-inquire |
Creative Biolabs |
inquire |
Ask for price |
Description: African swine fever virus (strain Badajoz 1971 Vero-adapted) Probable mRNA-capping enzyme (Ba71V-101), partial, recombinant protein from E. coli. (Uniprot ID: P32094) |
CNOT2 (Ab-101) Conjugated Antibody |
C33291 |
SAB |
100ul |
EUR 397 |
CAL-101 (Idelalisib, GS-1101) |
A3005-100 |
ApexBio |
100 mg |
EUR 357 |
Description: CAL-101 (Idelalisib, GS-1101), is a p110? selective phosphatidylinositol-3-kinase inhibitor in a kinome-wide screen using purified enzymes and in cell-based PI3K isoform-specific assays. |
CAL-101 (Idelalisib, GS-1101) |
A3005-20 |
ApexBio |
20 mg |
EUR 177 |
Description: CAL-101 (Idelalisib, GS-1101), is a p110? selective phosphatidylinositol-3-kinase inhibitor in a kinome-wide screen using purified enzymes and in cell-based PI3K isoform-specific assays. |
CAL-101 (Idelalisib, GS-1101) |
A3005-5 |
ApexBio |
5 mg |
EUR 108 |
Description: CAL-101 (Idelalisib, GS-1101), is a p110? selective phosphatidylinositol-3-kinase inhibitor in a kinome-wide screen using purified enzymes and in cell-based PI3K isoform-specific assays. |
CAL-101 (Idelalisib, GS-1101) |
A3005-5.1 |
ApexBio |
10 mM (in 1mL DMSO) |
EUR 119 |
Description: CAL-101 (Idelalisib, GS-1101), is a p110? selective phosphatidylinositol-3-kinase inhibitor in a kinome-wide screen using purified enzymes and in cell-based PI3K isoform-specific assays. |
CAL-101 (Idelalisib, GS-1101) |
A3005-50 |
ApexBio |
50 mg |
EUR 235 |
Description: CAL-101 (Idelalisib, GS-1101), is a p110? selective phosphatidylinositol-3-kinase inhibitor in a kinome-wide screen using purified enzymes and in cell-based PI3K isoform-specific assays. |
CAL-101 (Idelalisib, GS-1101) |
A3005-S |
ApexBio |
Evaluation Sample |
EUR 81 |
Description: CAL-101 (Idelalisib, GS-1101), is a p110? selective phosphatidylinositol-3-kinase inhibitor in a kinome-wide screen using purified enzymes and in cell-based PI3K isoform-specific assays. |
hsa-miR-101 miRNA Inhibitor |
MIH01030 |
ABM |
2 x 5.0 nmol |
EUR 176 |
hsa-miR-101 miRNA Antagomir |
MNH01030 |
ABM |
2 x 5.0 nmol |
EUR 329 |
hsa-miR-101-3p Primers |
MPH02060 |
ABM |
150 ul / 10 uM |
EUR 121 |
hsa-miR-101-5p Primers |
MPH02061 |
ABM |
150 ul / 10 uM |
EUR 121 |
Anti-hCD50 Antibody (Monoclonal, 101-1D2) |
M03651 |
BosterBio |
100 ug |
EUR 397 |
Description: Mouse Monoclonal hCD50 Antibody (Monoclonal, 101-1D2). Validated in Flow Cytometry and tested in Human. |
Tumor Susceptibility Gene 101 (TSG101) Antibody |
20-abx126743 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Tumor Susceptibility Gene 101 (TSG101) Antibody |
20-abx116380 |
Abbexa |
|
|
|
Sulforhodamine 101 cadaverine (Texas Red cadaverine) |
90109 |
Biotium |
5MG |
EUR 204 |
Description: Minimum order quantity: 1 unit of 5MG |
2-Sulforhodamine 101 cadaverine, trifluoroacete salt |
90115 |
Biotium |
5MG |
EUR 342 |
Description: Minimum order quantity: 1 unit of 5MG |
4-Sulforhodamine 101 cadaverine, trifluoroacete salt |
90116 |
Biotium |
5MG |
EUR 342 |
Description: Minimum order quantity: 1 unit of 5MG |
MTS-sulforhodamine 101 (MTS-texas red) |
91048 |
Biotium |
5MG |
EUR 244 |
Description: Minimum order quantity: 1 unit of 5MG |
CD50 (ICAM-3)(101-1D2) Antibody |
BNC040177-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD50 (ICAM-3)(101-1D2), CF405S conjugate, Concentration: 0.1mg/mL |
Construction of a quencher-free cascade amplification system for highly specific and sensitive detection of serum circulating miRNAs.
The reduced complexity, cost, and training requirements of OLA-Simple could improve access to HIVDR testing in low-resource settings and potentially allow same-day selection of appropriate antiretroviral therapy. FUND: USA National Institutes of Health R01; the Clinical and Retrovirology Research Core and the Molecular Profiling and Computational Biology Core of the UW CFAR; Seattle Children’s Research Institute; UW Holloman Innovation Challenge Award; Pilcher Faculty Fellowship.