OLA-Simple: A software-guided HIV-1 drug resistance test for low-resource laboratories.

HIV drug resistance (HIVDR) testing can assist clinicians in selecting treatments. However, high complexity and cost of genotyping assays limit routine testing in settings where HIVDR prevalence has reached high levels.

The oligonucleotide ligation assay (OLA)-Simple kit was developed for detection of HIVDR against first-line non-nucleoside/nucleoside reverse transcriptase inhibitors and validated on 672 codons (168 specimens) from subtypes A, B, C, D, and AE.

The kit uses dry reagents to facilitate assay setup, lateral flow devices for visual HIVDR detections, and in-house software with an interface for guiding users and analyzing results.HIVDR analysis of specimens by OLA-Simple compared to Sanger sequencing revealed 99.6 ± 0.3% specificity and 98.2 ± 0.9% sensitivity, and compared to high-sensitivity assays, 99.6 ± 0.6% specificity and 86.2 ± 2.5% sensitivity, with 2.6 ± 0.9% indeterminate results. OLA-Simple was performed more rapidly compared to Sanger sequencing (<4 h vs. 35-72 h).

Forty-one untrained volunteers blindly tested two specimens each with 96.8 ± 0.8% accuracy.OLA-Simple compares favorably with HIVDR genotyping by Sanger and sensitive comparators. Instructional software enabled inexperienced, first-time users to perform the assay with high accuracy.

MG-101
HY-18964 50mg
EUR 601

UCPH-101
HY-10914 100mg
EUR 2184

ULK-101
HY-114490 10mg
EUR 911

Sulforhodamine 101
HY-101878 25mg
EUR 119

CUDC-101
HY-10223 50mg
EUR 337

Sulforhodamine 101
GT6456-100MG 100 mg
EUR 158

Sulforhodamine 101
80101 500MG
EUR 236
Description: Minimum order quantity: 1 unit of 500MG

Trap 101
B7084-10 10 mg
EUR 373

Trap 101
B7084-50 50 mg
EUR 1363

UFP-101
B5128-1 1 mg
EUR 583

LOXO-101
B6176-100 100 mg
EUR 696
Description: IC50: low nanomolar range for inhibition of all TRK family membersLOXO-101 is a tropomyosin receptor kinases (TRK) inhibitor.The TRK family of neurotrophin receptors and their neurotrophin ligands regulate neuron growth, differentiation and survival.

LOXO-101
B6176-25 25 mg
EUR 290
Description: IC50: low nanomolar range for inhibition of all TRK family membersLOXO-101 is a tropomyosin receptor kinases (TRK) inhibitor.The TRK family of neurotrophin receptors and their neurotrophin ligands regulate neuron growth, differentiation and survival.

LOXO-101
B6176-5 5 mg
EUR 128
Description: IC50: low nanomolar range for inhibition of all TRK family membersLOXO-101 is a tropomyosin receptor kinases (TRK) inhibitor.The TRK family of neurotrophin receptors and their neurotrophin ligands regulate neuron growth, differentiation and survival.

GlyH-101
B1087-10 10 mg
EUR 195
Description: GlyH-101 is a cell-permeable glycinyl hydrazone compound that blocks CFTR with Ki of 1.4 uM.

GlyH-101
B1087-5.1 10 mM (in 1mL DMSO)
EUR 148
Description: GlyH-101 is a cell-permeable glycinyl hydrazone compound that blocks CFTR with Ki of 1.4 uM.

GlyH-101
B1087-50 50 mg
EUR 659
Description: GlyH-101 is a cell-permeable glycinyl hydrazone compound that blocks CFTR with Ki of 1.4 uM.

ARP 101
A4433-5 5 mg
EUR 334
Description: ARP 101 is a selective MMP-2 inhibitor with IC50 value of 0.81nM.

AT-101
A3196-10 10 mg
EUR 171
Description: AT101, a natural product from cottonseed with a BH3-mimetic structure, was identified as a small molecule inhibitor of Bcl-2/Bcl-xL/Mcl-1 that potently induces apoptosis in various cancer cell lines [1].

AT-101
A3196-50 50 mg
EUR 432
Description: AT101, a natural product from cottonseed with a BH3-mimetic structure, was identified as a small molecule inhibitor of Bcl-2/Bcl-xL/Mcl-1 that potently induces apoptosis in various cancer cell lines [1].

Sulforhodamine 101
B7762-100 100 mg
EUR 137

Sulforhodamine 101
B7762-250 250 mg
EUR 257

Sulforhodamine 101
B7762-500 500 mg
EUR 390

AS 101
B7830-5 5 mg
EUR 171
Description: Ammonium tri-chloro(dioxoethylene-O,O?-)tellurate (AS-101), an anti?IL-10 compound, is a nontoxic immunomodulator.AS101 (0.43222.5 ?g/ml) decreased IL-10 production in a dose-dependent manner.

CUDC-101
A4092-10 10 mg
EUR 137
Description: CUDC-101 is a multitargeted inhibitor of histone deacetylase. It is designed to directly inhibit epidermal growth factor receptor (EGFR) and HER2 as well as class I and class II HDACs.

CUDC-101
A4092-200 200 mg
EUR 943
Description: CUDC-101 is a multitargeted inhibitor of histone deacetylase. It is designed to directly inhibit epidermal growth factor receptor (EGFR) and HER2 as well as class I and class II HDACs.

CUDC-101
A4092-5.1 10 mM (in 1mL DMSO)
EUR 158
Description: CUDC-101 is a multitargeted inhibitor of histone deacetylase. It is designed to directly inhibit epidermal growth factor receptor (EGFR) and HER2 as well as class I and class II HDACs.

CUDC-101
A4092-50 50 mg
EUR 386
Description: CUDC-101 is a multitargeted inhibitor of histone deacetylase. It is designed to directly inhibit epidermal growth factor receptor (EGFR) and HER2 as well as class I and class II HDACs.

CUDC-101
A4092-S Evaluation Sample
EUR 81
Description: CUDC-101 is a multitargeted inhibitor of histone deacetylase. It is designed to directly inhibit epidermal growth factor receptor (EGFR) and HER2 as well as class I and class II HDACs.

UCF 101
C4437-10 10 mg
EUR 186
Description: IC50: 9.5 ?MUCF 101 is an inhibitor of Omi/HtrA2. Omi/HtrA2, a mitochondria serine protease with close homology to bacterial HtrA chaperones, is released from the mitochondria in response to apoptotic stimuli.

UCF 101
C4437-25 25 mg
EUR 354
Description: IC50: 9.5 ?MUCF 101 is an inhibitor of Omi/HtrA2. Omi/HtrA2, a mitochondria serine protease with close homology to bacterial HtrA chaperones, is released from the mitochondria in response to apoptotic stimuli.

UCF 101
C4437-5 5 mg
EUR 145
Description: IC50: 9.5 ?MUCF 101 is an inhibitor of Omi/HtrA2. Omi/HtrA2, a mitochondria serine protease with close homology to bacterial HtrA chaperones, is released from the mitochondria in response to apoptotic stimuli.

LOXO-101
B2291-25
EUR 631

LOXO-101
B2291-5
EUR 196

KHS-101
B2415-25
EUR 588

KHS-101
B2415-5
EUR 185

CUDC-101
1966-25
EUR 555

CUDC-101
1966-5
EUR 202

AT-101
2380-25
EUR 506

AT-101
2380-5
EUR 164

miRZip-101 anti-miR-101 microRNA construct
MZIP101-PA-1 Bacterial Streak
EUR 684

ECOS 101 (DH5a
FYE607-10VL 100 µl x 10 vials Ask for price

ECOS 101 (DH5a
FYE607-80VL 100 µl x 10 vials Ask for price

ECOS 101 (DH5a
FYE608-10VL 100 µl x 10 vials Ask for price

ECOS 101 (DH5a
FYE608-80VL 100 µl x 10 vials Ask for price

ECOS 101 (DH5a
FYE609-10VL 100 µl x 10 vials Ask for price

ECOS 101 (DH5a
FYE609-80VL 100 µl x 10 vials Ask for price

KHS 101 hydrochloride
B7717-10 10 mg
EUR 318

KHS 101 hydrochloride
B7717-50 50 mg
EUR 1162

LOXO-101 sulfate
B2545-25
EUR 414

LOXO-101 sulfate
B2545-5
EUR 196

LB (Luria)
101 500 g
EUR 108

H1F0 (Ab-101) Antibody
1-CSB-PA010087OA101nme1HU
  • EUR 332.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against H1F0 (Ab-101). Recognizes H1F0 (Ab-101) from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF, ChIP; Recommended dilution: WB:1:200-1:2000, IHC:1:20-1:200, IF:1:50-1:200

HIST1H2AG (Ab-101) Antibody
1-CSB-PA010389PA101nogHU
  • EUR 332.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against HIST1H2AG (Ab-101). Recognizes HIST1H2AG (Ab-101) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

CNOT2 (Ab-101) Antibody
CSB-PA951939-
EUR 335
Description: A polyclonal antibody against CNOT2 (Ab-101). Recognizes CNOT2 (Ab-101) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

CNOT2 (Ab-101) Antibody
CSB-PA951939-100ul 100ul
EUR 316
Description: A polyclonal antibody against CNOT2 (Ab-101). Recognizes CNOT2 (Ab-101) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

Tsg 101 Polyclonal Antibody
ES7453-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Tsg 101 from Human/Mouse/Rat/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Tsg 101 Polyclonal Antibody
ES7453-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Tsg 101 from Human/Mouse/Rat/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

CNOT2 (Ab-101) Antibody
33291-100ul 100ul
EUR 252

CNOT2 (Ab-101) Antibody
33291-50ul 50ul
EUR 187

Tsg 101 Polyclonal Antibody
ABP56454-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Tsg 101 from Human, Mouse, Rat, Monkey. This Tsg 101 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Tsg 101 at AA range: 250-330

Tsg 101 Polyclonal Antibody
ABP56454-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Tsg 101 from Human, Mouse, Rat, Monkey. This Tsg 101 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Tsg 101 at AA range: 250-330

Tsg 101 Polyclonal Antibody
ABP56454-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Tsg 101 from Human, Mouse, Rat, Monkey. This Tsg 101 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Tsg 101 at AA range: 250-330

Anti-Tsg 101 antibody
STJ96125 200 µl
EUR 197
Description: Rabbit polyclonal to Tsg 101.

hsa-miR-101 Primers
MPH01016 150 ul / 10 uM
EUR 121

CAL-101 (GS-1101)
2613-25
EUR 370

CAL-101 (GS-1101)
2613-5
EUR 131

CFTR Inhibitor, GlyH-101
2863-25
EUR 756

CFTR Inhibitor, GlyH-101
2863-5
EUR 229

Monoclonal SDC1 / Syndecan 1 / CD138 Antibody (clone DL-101), Clone: DL-101
AMR09843G 0.05mg
EUR 484
Description: A Monoclonal antibody against Human SDC1 / Syndecan 1 / CD138 (clone DL-101). The antibodies are raised in Mouse and are from clone DL-101. This antibody is applicable in IHC-P, IHC-Fr, Flo

Recombinant ASFV Pret-101 Protein
VAng-2391Lsx-inquire inquire Ask for price
Description: African swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996) Uncharacterized protein G1340L (Pret-101), partial, recombinant protein from E. coli. (Uniprot ID: P0CA42)

Transmembrane Protein 101 (TMEM101) Antibody
20-abx015121
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Apo B48 / 101 Antibody (Biotin)
abx023237-1ml 1 ml
EUR 787

Tumor Susceptibility Gene 101 Protein
20-abx262882
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg

Testis Expressed 101 (TEX101) Antibody
abx238605-100ug 100 ug
EUR 551

Transmembrane Protein 101 (TMEM101) Antibody
abx331212-100ul 100 ul
EUR 425

Transmembrane Protein 101 (TMEM101) Antibody
20-abx325447
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Sulforhodamine 101 (Texas-Red) Phalloidin
00033 300EU
EUR 345
Description: Minimum order quantity: 1 unit of 300EU

Recombinant ASFV Ken-101 Protein
VAng-0862Lsx-inquire inquire Ask for price
Description: African swine fever virus (isolate Pig/Kenya/KEN-50/1950) Uncharacterized protein G1340L (Ken-101), partial, recombinant protein from E. coli. (Uniprot ID: P0CA40)

Recombinant ASFV Ba71V-101 Protein
VAng-2017Lsx-inquire inquire Ask for price
Description: African swine fever virus (strain Badajoz 1971 Vero-adapted) Probable mRNA-capping enzyme (Ba71V-101), partial, recombinant protein from E. coli. (Uniprot ID: P32094)

CNOT2 (Ab-101) Conjugated Antibody
C33291 100ul
EUR 397

CAL-101 (Idelalisib, GS-1101)
A3005-100 100 mg
EUR 357
Description: CAL-101 (Idelalisib, GS-1101), is a p110? selective phosphatidylinositol-3-kinase inhibitor in a kinome-wide screen using purified enzymes and in cell-based PI3K isoform-specific assays.

CAL-101 (Idelalisib, GS-1101)
A3005-20 20 mg
EUR 177
Description: CAL-101 (Idelalisib, GS-1101), is a p110? selective phosphatidylinositol-3-kinase inhibitor in a kinome-wide screen using purified enzymes and in cell-based PI3K isoform-specific assays.

CAL-101 (Idelalisib, GS-1101)
A3005-5 5 mg
EUR 108
Description: CAL-101 (Idelalisib, GS-1101), is a p110? selective phosphatidylinositol-3-kinase inhibitor in a kinome-wide screen using purified enzymes and in cell-based PI3K isoform-specific assays.

CAL-101 (Idelalisib, GS-1101)
A3005-5.1 10 mM (in 1mL DMSO)
EUR 119
Description: CAL-101 (Idelalisib, GS-1101), is a p110? selective phosphatidylinositol-3-kinase inhibitor in a kinome-wide screen using purified enzymes and in cell-based PI3K isoform-specific assays.

CAL-101 (Idelalisib, GS-1101)
A3005-50 50 mg
EUR 235
Description: CAL-101 (Idelalisib, GS-1101), is a p110? selective phosphatidylinositol-3-kinase inhibitor in a kinome-wide screen using purified enzymes and in cell-based PI3K isoform-specific assays.

CAL-101 (Idelalisib, GS-1101)
A3005-S Evaluation Sample
EUR 81
Description: CAL-101 (Idelalisib, GS-1101), is a p110? selective phosphatidylinositol-3-kinase inhibitor in a kinome-wide screen using purified enzymes and in cell-based PI3K isoform-specific assays.

hsa-miR-101 miRNA Inhibitor
MIH01030 2 x 5.0 nmol
EUR 176

hsa-miR-101 miRNA Antagomir
MNH01030 2 x 5.0 nmol
EUR 329

hsa-miR-101-3p Primers
MPH02060 150 ul / 10 uM
EUR 121

hsa-miR-101-5p Primers
MPH02061 150 ul / 10 uM
EUR 121

Anti-hCD50 Antibody (Monoclonal, 101-1D2)
M03651 100 ug
EUR 397
Description: Mouse Monoclonal hCD50 Antibody (Monoclonal, 101-1D2). Validated in Flow Cytometry and tested in Human.

Tumor Susceptibility Gene 101 (TSG101) Antibody
20-abx126743
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Tumor Susceptibility Gene 101 (TSG101) Antibody
20-abx116380
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Sulforhodamine 101 cadaverine (Texas Red cadaverine)
90109 5MG
EUR 204
Description: Minimum order quantity: 1 unit of 5MG

2-Sulforhodamine 101 cadaverine, trifluoroacete salt
90115 5MG
EUR 342
Description: Minimum order quantity: 1 unit of 5MG

4-Sulforhodamine 101 cadaverine, trifluoroacete salt
90116 5MG
EUR 342
Description: Minimum order quantity: 1 unit of 5MG

MTS-sulforhodamine 101 (MTS-texas red)
91048 5MG
EUR 244
Description: Minimum order quantity: 1 unit of 5MG

CD50 (ICAM-3)(101-1D2) Antibody
BNC040177-100 100uL
EUR 199
Description: Primary antibody against CD50 (ICAM-3)(101-1D2), CF405S conjugate, Concentration: 0.1mg/mL


Construction of a quencher-free cascade amplification system for highly specific and sensitive detection of serum circulating miRNAs.

The reduced complexity, cost, and training requirements of OLA-Simple could improve access to HIVDR testing in low-resource settings and potentially allow same-day selection of appropriate antiretroviral therapy. FUND: USA National Institutes of Health R01; the Clinical and Retrovirology Research Core and the Molecular Profiling and Computational Biology Core of the UW CFAR; Seattle Children’s Research Institute; UW Holloman Innovation Challenge Award; Pilcher Faculty Fellowship.