Ernest Stevens

Genotyping cancer-associated genes in chordoma identifies mutations in oncogenes and areas of chromosomal loss involving CDKN2A, PTEN, and SMARCB1.

Genotyping cancer-associated genes in chordoma identifies mutations in oncogenes and areas of chromosomal loss involving CDKN2A, PTEN, and SMARCB1.

The molecular mechanisms underlying chordoma pathogenesis are unknown. We due to this fact sought to determine novel mutations to higher perceive chordoma biology and to doubtlessly determine therapeutic targets. Given the comparatively excessive prices of entire genome sequencing, we carried out a centered genetic evaluation utilizing matrix-assisted laser desorption/ionization-time of flight mass spectrometer (Sequenom iPLEX genotyping). We examined 865 hotspot …

Genotyping cancer-associated genes in chordoma identifies mutations in oncogenes and areas of chromosomal loss involving CDKN2A, PTEN, and SMARCB1. Read More »

Novel Multiplex and Loop-Mediated Isothermal Amplification (LAMP) Assays for Rapid Species and Mating-Type Identification of Oculimacula acuformis and O. yallundae (Causal Agents of Cereal Eyespot), and Application for Detection of Ascospore Dispersal and in planta Use

Novel Multiplex and Loop-Mediated Isothermal Amplification (LAMP) Assays for Rapid Species and Mating-Type Identification of Oculimacula acuformis and O. yallundae (Causal Agents of Cereal Eyespot), and Application for Detection of Ascospore Dispersal and in planta Use

Eyespot, attributable to the associated fungal pathogens Oculimacula acuformis (OA) and O. yallundae (OY), is a vital cereal stem-base illness in temperate elements of the world. Both species are dispersed primarily by splash-dispersed conidia however are additionally recognized to bear sexual replica yielding apothecia containing ascospores. Field prognosis of eyespot could be difficult with different …

Novel Multiplex and Loop-Mediated Isothermal Amplification (LAMP) Assays for Rapid Species and Mating-Type Identification of Oculimacula acuformis and O. yallundae (Causal Agents of Cereal Eyespot), and Application for Detection of Ascospore Dispersal and in planta Use Read More »

Molecular Epidemiology of Giardia Infections in the Genomic Era

Molecular Epidemiology of Giardia Infections in the Genomic Era

Giardia duodenalis is a serious gastrointestinal parasite of people and animals throughout the globe. It can also be of curiosity from an evolutionary perspective because it possesses many options which can be distinctive amongst the eukaryotes, together with its distinctive binucleate cell construction. While genomic evaluation of a small quantity of isolates has supplied worthwhile …

Molecular Epidemiology of Giardia Infections in the Genomic Era Read More »

Versatile in vitro assay to recognize Cas9-induced mutations

Versatile in vitro assay to recognize Cas9-induced mutations

The discovery of CRISPR/Cas9 has revolutionized molecular biology, and its affect on plant biotechnology and plant breeding can’t be over-estimated. In many plant species, its utility for mutagenesis is now a routine procedure–if appropriate goal websites, adequate expression of the Cas9 protein, and functioning sgRNAs are mixed. sgRNAs differ in their effectivity, relying on parameters which are …

Versatile in vitro assay to recognize Cas9-induced mutations Read More »

Influence of the kappa casein genotype on the technological properties of cow milk of Simmental and Alatau breeds

Influence of the kappa casein genotype on the technological properties of cow milk of Simmental and Alatau breeds

The fundamental goal of dairy farming is to acquire extremely productive animals that produce milk with excessive protein content material and have good technological properties. Currently, with the improvement of molecular genetics and molecular biology, it’s doable to determine genes straight or not directly related to the milk manufacturing of animals. The identification of variants of such genes which …

Influence of the kappa casein genotype on the technological properties of cow milk of Simmental and Alatau breeds Read More »

An overview of coronaviruses including the SARS-2 coronavirus - Molecular biology, epidemiology and clinical implications.

An overview of coronaviruses including the SARS-2 coronavirus – Molecular biology, epidemiology and clinical implications.

Coronavirus infections have emerged as epidemic and pandemic threats in final 20 years. After the H1N1 influenza pandemic in 2009, not too long ago recognized novel betacoronavirus or extreme acute respiratory syndrome coronavirus (SARS-CoV)-2 has unfold throughout 203 international locations and territories in all 5 main continents. World Health Organization (WHO) declared this as a …

An overview of coronaviruses including the SARS-2 coronavirus – Molecular biology, epidemiology and clinical implications. Read More »

Advances in the Knowledge of the Molecular Biology of Glioblastoma and Its Impact in Patient Diagnosis, Stratification, and Treatment.

Advances in the Knowledge of the Molecular Biology of Glioblastoma and Its Impact in Patient Diagnosis, Stratification, and Treatment.

Gliomas are the most typical major mind tumors in adults. They come up in the glial tissue and primarily happen in the mind. Low-grade tumors of World Health Organization (WHO) grade II are likely to progress to high-grade gliomas of WHO grade III and, ultimately, glioblastoma of WHO grade IV, which is the most typical …

Advances in the Knowledge of the Molecular Biology of Glioblastoma and Its Impact in Patient Diagnosis, Stratification, and Treatment. Read More »

Cryptosporidium propidium monoazide-PCR, a molecular biology-based technique for genotyping of viable Cryptosporidium oocysts.

Cryptosporidium propidium monoazide-PCR, a molecular biology-based technique for genotyping of viable Cryptosporidium oocysts.

Cryptosporidium is a crucial waterborne protozoan parasite that may trigger extreme diarrhea and demise within the immunocompromised. The present strategies used to observe for Cryptosporidium oocysts in water are the microscopy-based USEPA strategies 1622 and 1623. These strategies assess complete ranges of oocysts in supply waters, however don’t decide oocyst viability or genotype. Recently, propidium …

Cryptosporidium propidium monoazide-PCR, a molecular biology-based technique for genotyping of viable Cryptosporidium oocysts. Read More »