Versatile in vitro assay to recognize Cas9-induced mutations

Versatile in vitro assay to recognize Cas9-induced mutations

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The discovery of CRISPR/Cas9 has revolutionized molecular biology, and its affect on plant biotechnology and plant breeding can’t be over-estimated. In many plant species, its utility for mutagenesis is now a routine procedure–if appropriate goal websites, adequate expression of the Cas9 protein, and functioning sgRNAs are mixed. sgRNAs differ in their effectivity, relying on parameters which are

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